Plasmid_Backbone

Part:BBa_K4201013

Designed by: David Birkhaeuser   Group: iGEM22_CU-Boulder   (2022-10-11)


Amp BsaI

This Ampicillin Backbone conveys ampicillin resistance in transformed bacteria. The backbone also contains a Blue/White marker which is flanked by BsaI cut sites. This marker is excised during Golden Gate gene assembly and replaced by a gene of interest, allowing blue/white selection of transformed colonies.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 304
    Illegal XbaI site found at 277
    Illegal PstI site found at 265
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 304
    Illegal PstI site found at 265
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 304
    Illegal BamHI site found at 283
    Illegal XhoI site found at 1641
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 304
    Illegal XbaI site found at 277
    Illegal PstI site found at 265
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 304
    Illegal XbaI site found at 277
    Illegal PstI site found at 265
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 701
    Illegal BsaI.rc site found at 17


[edit]
Categories
Parameters
None